Experimental Disease in Vivo and In Vitro Models

Experimental animal models play a crucial role in advancing scientific research across disciplines such as medicine, biology, and pharmacology. They provide an indispensable bridge between in vitro experiments and human clinical studies, allowing researchers to investigate physiological and pathological processes in living systems that closely resemble humans. By studying disease mechanisms in animals, scientists can gain valuable insights into molecular, cellular, and systemic functions, which can then be translated into human health applications. Despite ongoing efforts to reduce animal use, their role in translational research remains fundamental. The implementation of alternative models-including in vitro systems, organ-on-chip technologies, and computational simulations-supports the principles of the 3Rs (Replacement, Reduction, and Refinement) while ensuring reliable, reproducible, and ethical biomedical research.